Figure 6: Tumour-derived sST2 suppresses the expression of Th1- and Th2-related genes in tumours. | Nature Communications

Figure 6: Tumour-derived sST2 suppresses the expression of Th1- and Th2-related genes in tumours.

From: Soluble IL-33 receptor sST2 inhibits colorectal cancer malignant growth by modifying the tumour microenvironment

Figure 6

(a) IκBα phosphorylation in the tumours. The lysates of NM11-shCont, NM11-shsST2, LuM1-VC and LuM1-sST2 tumours were subjected to immunoblot analysis of p-IκBα and total IκBα (n=3). The lysates of NIH3T3 cells treated with TNF-α (10 ng ml−1) were used as a positive control. (b) Upregulation of Th1- and Th2-related genes in NM11-shsST2 tumours. Gene expression profiling of cytokines and chemokines between NM11-shsST2 and NM11-shCont tumours was performed using a PCR array. (c) qRT-PCR analysis of Ifnγ, Il4 and Il13 mRNA expression in the indicated tumours (n=7). (d) Concentrations of IFN-γ, IL-4 and IL-13 in the indicated tumours (n=7). The data are shown as the mean±s.d. *P<0.05, **P<0.01, ***P<0.001 by Student’s t-test.

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