Figure 6: NPM1 dissociates from rRNA/rDNA after exposed to oxidized GSH in vitro.
From: A redox mechanism underlying nucleolar stress sensing by nucleophosmin
SPR analysis of the interaction of NPM1-C70 (WT or C275S, as analyte) and G-quadruplexes (rDNA (upper) or rRNA (bottom), as ligand). Different colourful traces referred to increasing analyte concentrations (μg ml−1). The dissociation constant (KD) values were calculated with a one-site steady state binding model (see Supplementary Fig. 5g and Table 1). GSSG, oxidized GSH. G4, G-quadruplex.
