Table 1 Effect of cleavage on the potency of CGRP-based protease sensors.

From: Molecular imaging with engineered physiology

Construct

Log(EC50)

 

Uncleaved

Cleaved

CGRP-NH2

NA

−10.3

CGRP-G

NA

−8.6

Sensor (1)

−9.9

−10.8

Sensor (2)

<−5

−6.0

Sensor (3)

<−5

−6.4

Sensor (4)

<−5

−6.4

Sensor (5)

−8.2

−10.4

  1. NA, not applicable.
  2. Log(EC50) values obtained by dose–response curve measurement from sensors (1–5) from Fig. 3 incubated with or without corresponding proteases: (1) with 5 ng μl−1 of human FAP; (2) with 0.1 U μl−1 of TEV protease; (3) with 2 pg μl−1 of enterokinase light chain; (4) with 23 ng ul−1 and (5) with 11.5 ng μl−1 of human CASP3. s.e.m. values were all <0.1 (n=7).
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