Figure 8: Molecular effects of TUG1 inhibition in mouse xenograft model.
From: Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment
(a) RNA-FISH analysis of TUG1 (red) and Notch1 (green) in tumour cells of CTRL-DDS (upper panels) and TUG1-DDS-treated mice (bottom panels). (b) Immunostaining of CD15 (red) and Nestin-EGFP (green) in CTRL-DDS (upper panels) and TUG1-DDS-treated tumour (bottom panels). Asterisk indicates blood vessel in CTRL-DDS treated mice. Tumour areas are surrounded with the dashed line in TUG1-DDS-treated mice. Scale bars, 100 μm. (c) Expression levels of miR-145, SOX2, MYC and TUG1 target genes (BDNF, NGF and NTF3) were examined by qPCR in tumour cells derived from the mouse xenograft. Relative expression levels compared with that in the CTRL-DDS-treated tumour are indicated on the y-axis (n=4). Error bars indicate s.e.m. *P<0.001, Student’s t-test. (d) Immunostaining of NGF in CTRL-DDS (left) or TUG1-DDS-treated tumour (right) at 4 weeks post treatment. Tumour areas are surrounded with the dashed line in TUG1-DDS-treated mice. Scale bars, 1 mm.
