Figure 3: siRNA-mediated CDA depletion partially reproduces BS phenotype. | Nature Communications

Figure 3: siRNA-mediated CDA depletion partially reproduces BS phenotype.

From: Pyrimidine pool imbalance induced by BLM helicase deficiency contributes to genetic instability in Bloom syndrome

Figure 3

(a) Percentage of metaphases with n SCE per chromosome from GFP-BLM cells transfected with the indicated siRNAs. In all, 40–50 metaphases from three independent experiments were analysed for each condition. CDA levels were assessed by immunoblotting. (b) Percentage of metaphases with n SCEs per chromosome from HeLaV-siCtrl and HeLash-siBLM cells transiently transfected with the indicated siRNAs. CDA protein levels were assessed by immunoblotting. About 40–50 metaphases from two independent experiments were analysed for each condition. (c) Replication fork velocity in GFP-BLM and BS cells transfected with the indicated siRNAs. Error bars represent the range of two independent experiments. Mann–Whitney statistical tests have been performed on the total number of forks analysed from the two experiments (GFP-BLM siCtrl, n=312; GFP-BLM siCDA, n=452; BS siCtrl: n=459; and BS siCDA, n=467). Asterisks indicate a P-value <0.00001.

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