Figure 4: The BS phenotype is partially complemented by dU or T. | Nature Communications

Figure 4: The BS phenotype is partially complemented by dU or T.

From: Pyrimidine pool imbalance induced by BLM helicase deficiency contributes to genetic instability in Bloom syndrome

Figure 4

(a) SCE frequencies in BS and GFP-BLM cell lines untreated or treated with dU or T (about 1,800 chromosomes analysed for each condition). Error bars represent the s.e.m. of three independent experiments. The significance of differences was assessed by Student's t-tests. (b) Replication fork velocity in GFP-BLM and BS cell lines untreated or treated with dU. Error bars represent the range of three independent experiments. Mann–Whitney statistical tests have been performed on the total number of forks analysed from the three experiments (GFP-BLM, n=530; GFP-BLM+dU, n=642; BS, n=858; and BS+dU, n=440). Asterisks indicate a P-value <0.00001. (c) Percentage of asymmetric replication bubbles in BS and GFP-BLM cell lines untreated or treated with dU, and examples of symmetric and asymmetric bubbles (70–130 replication bubbles analysed for each condition). CldU, chlorodeoxyuridine; IdU, iododeoxyuridine. Error bars represent the range of two independent experiments.

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