Figure 5: Wnt5a increases intracellular calcium and osmotic water permeability in isolated CCD of mouse kidneys.
From: Wnt5a induces renal AQP2 expression by activating calcineurin signalling pathway
(a) The increase of Fluo4 intensity. (Upper) Wnt5a (500 ng ml−1) was added to the CCD cells dissected from mouse kidneys. The time-course of Fluo4 fluorescence intensity is shown. The x axis indicates time, and the y axis indicates Fluo4 intensity. (Lower) Representative confocal images at the indicated time (black arrow) are shown. Scale bars, 50 μm. TAL indicates thick ascending limb. (b) Pf in the mouse CCD. Wnt5a (500 ng ml−1) were added to isolated CCD tubules for 1 h. After Wnt5a washout, dDAVP (1 nM) was added to CCD for 15 min. Each value is an average of triplicate assays. P.C. indicates positive control. Mean values±s.e. were determined from six experiments. Student's t-test, *P<0.05, **P<0.01.
