Figure 3: In vivo CO reduction as a secondary metabolic pathway.
From: The in vivo hydrocarbon formation by vanadium nitrogenase follows a secondary metabolic pathway
(a–d) Secondary electron images (a,c) and secondary ion (13C− and 12C−) images (b,d) derived from nanoSIMS analysis of A. vinelandii cells expressing V-nitrogenase in the presence of 12CO (a,b) or 13CO (c,d). The nanoSIMS experiment was performed twice. Each time, data were collected at three different ROI in each sample. Representative nanoSIMS data are shown in a–d. (e) Average 13C abundance (13C/12C ratio) of A. vinelandii cells expressing V-nitrogenase in the presence of 12CO (blue) or 13CO (red). The average 13C/12C ratio of each sample was calculated based on data collected in three different ROI (n=9). (f) LC–MS analysis of acetyl-CoA formed by A. vinelandii cells expressing V-nitrogenase in the absence (left) and presence of 12CO (middle) or 13CO (right). Natural abundance (black), one (blue) and two (red) 13C-substituted acetyl-CoA molecules were traced at masses 810, 811 and 812, respectively. The LC–MS experiment was performed four times. Representative results are shown in f. For additional nanoSIMS experiments conducted in the presence of 15N2, see Supplementary Fig. 1. Scale bars, 1 μm (a,c).
