Figure 3: PPARγ controls expression of genes associated with FA uptake programme.

(a) Effects of silenced Pparg on the genes encoding the enzymes and transporters in fatty acid uptake programme in stimulated CD4⁺ T cells. Naive CD4⁺ T cells were electroporated with control or Srebf1 siRNA and cultured for 2 days, and qRT-PCR analyses of the indicated genes are shown. (b) qRT-PCR analyses of the indicated genes in activated CD4⁺ T cells with or without GW9662. (c) Effects of silenced Srebf1 on the genes encoding the enzymes in fatty acid biosynthesis programme in stimulated CD4⁺ T cells as in a. (d) ChIP assays were performed with anti-PPARγ at the promoter region of the target gene loci such as Fabp5, Ldlr, Plin2, Scarb1, Vldlr and Hprt from activated CD4⁺ T cells treated with DMSO (Control) and GW9662 (10 μM). (e) ChIP assays were performed with anti-SREBP1 at the promoter regions of Acaca, Fads2, Scd2 and Hprt from activated CD4⁺ T cells treated with DMSO (Control) and rapamycin (Rap.; 5 nM). Three technical replicates were performed for qRT-PCR (a–c) and ChIP qRT-PCR (d,e). Three independent experiments were performed with similar results (a–e).