Figure 7: Anabolic FA metabolism is essential for activation of human CD4⁺ T cells.

(a) qRT-PCR analyses of the relative expression of the genes encoding the enzymes and transporter in fatty acid uptake and lipolysis programmes in activated human CD4⁺ T cells 24 h after TCR stimulation in the presence or absence of GW9662 (10 μM). (**P<0.01, Mann–Whitney U test) (b) Representative profiles of e670 and Bodipy FLC16 in activated human CD4⁺ T cells in the presence or absence of GW9662. (c) Basal levels of OCR and ECAR of activated human CD4⁺ T cells for 48 h with TCR stimulation in the presence or absence of indicated doses of GW9662. (**P<0.01, Mann–Whitney U test, n=6 per group) (d) OCR of activated human CD4⁺ T cells 48 h after TCR stimulation with or without GW9662 or TOFA under basal conditions and in response to sequential treatment with Oligomycin, FCCP and Rotenone-Antimycin A. (e) ECAR of activated human CD4⁺ T cells 48 h after TCR stimulation with or without GW9662 or TOFA under basal conditions and in response to sequential treatment with Oligomycin, FCCP and Rotenone-Antimycin A as in d. (f) Representative plot of e670 proliferation dye in activated human CD4⁺ T cells with or without oleic acid treatment under normal or fatty acid-free conditions 48 h after TCR stimulation was shown. Three technical replicates were performed for qRT-PCR (a). Six technical replicates were performed for Seahorse assay (c–e). The mean values with s.d. are shown (a,c,d,e). **P<0.01. Three independent experiments were performed with similar results (a,b). Two independent experiments were performed with similar results (c–f).