Figure 5: Microarray of gene expression in growth plates of Ezh1/2 mice.
Expression microarray was performed in RNA isolated from tibial growth plate proliferative or hypertrophic zone of 3-day-old Ezh1/2 or wild-type mice using laser capture microdissection. In both zones, using a cutoff of P<0.001 and fold change>1.3, more genes were upregulated than downregulated in Ezh1/2 mice compared with wild type (a). Overlap of genes commonly upregulated (or commonly downregulated) between two zones in the Ezh1/2 mice were modest (b). Unsupervised two-way hierarchical clustering of the 12 samples from Ezh1/2 mice and wild-type mice (six samples each), using the 280 significant genes in the PZ (top panel) and 243 significant genes in the HZ (bottom panel). Relative expression was depicted by colour maps, with blue representing low expression, and red representing high expression. Scale bar, normalized log2-transformed expression (c). Canonical signalling pathway analysis by Ingenuity Pathway Analysis on the 280 PZ genes (top panel) and 243 HZ genes (bottom panel) (d). A subset of messenger RNAs that were found to be differentially expressed in Ezh1/2 versus wild-type mice were analysed by real-time PCR for confirmation (e). Error bars, ±s.e.m.
