Figure 1: TNFRSF14 expression and function on MCs.
(a) TNFRSF14, TNFRSF3 and TNFSF14 expression (black lines) on human mast cell line LAD2 cells and human mast cells derived in vitro from human peripheral blood CD34+ mononuclear cells (huPBCMCs). Shaded areas: isotype control. (b–d) Enhanced IgE-dependent responses upon engagement of huPBCMC TNFRSF14 by TNFSF14. Human (h) LAMP-1 MFI (b) and concentrations of hIL-8 (c) and hTNF-α (d) in the supernatants of IgE presensitized-huPBCMCs, with or without anti-IgE stimulation in the absence or presence of TNFSF14. Results are pooled from three independent experiments, from two donors. (e) Tnfrsf14+/+ mouse bone marrow-derived cultured MCs (BMCMCs), stained for TNFRSF14, TNFRSF3 and TNFSF14 (black lines). Shaded areas: isotype control. Red line indicates TNFRSF14 expression on BMCMCs from Tnfrsf14−/− mice. (f) FcɛRIα+ and CD117+ expression levels (MFI) from three independent cell cultures of Tnfrsf14+/+ (black columns) and Tnfrsf14−/− (red columns) BMCMCs. (g–n) LAMP-1 MFI, production of histamine, TNF-α (early ‘pre-stored’, panel (i), later ‘de novo synthesized’, panel (l)), LTC4, LTE4, IL-6 and IL-13 were measured in the supernatants of IgE presensitized-Tnfrsf14+/+ BMCMCs, with or without Ag stimulation with or without TNFSF14. Results are pooled from at least four independent experiments, each of which gave similar results. The data in (b–d,f–n) (shown as mean+s.e.m.) were assessed for statistical significance using a two-tailed Student’s t-test. Asterisks indicate statistical significance of differences between non-Ag-treated and corresponding Ag-treated groups; daggers indicate statistical significance between indicated groups. * or †P<0.05; ** or ††P<0.01; ***P<0.001.
