Figure 2: TNFSF14 enhances IgE-dependent signalling in MCs. | Nature Communications

Figure 2: TNFSF14 enhances IgE-dependent signalling in MCs.

From: A TNFRSF14-FcɛRI-mast cell pathway contributes to development of multiple features of asthma pathology in mice

Figure 2

(a) Detection of phosphorylated Akt, PKC-δ, Mek, Erk1/2 and pulled-down Ras, 0, 10, 30, 60 min after stimulation with TNFSF14 in IgE-presensitized Tnfrsf14+/+ BMCMCs in the absence of Ag stimulation. Phorbol 12-myristate 13-acetate/Ionomycin (PMA/I) stimulation (10 min): positive control for mast cell activation. The blots were stripped and reprobed with antibodies recognizing total levels of Akt, PKC-δ, Mek and Erk1/2 for loading control. Loading control for Ras was collected from total BMCMC lysate before glutathione S-transferase (GST)-pulldown. (b) Detection of phospho-proteins as in (a) 0, 10, 30, 60 min after Ag stimulation or Ag/TNFSF14 co-stimulation of Tnfrsf14+/+ BMCMCs. All the blots were stripped and reprobed as in a. Loading control for Ras was collected as in a. Results shown are representative of three independent experiments, each of which gave similar results.

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