Figure 6: Passive transfer of Ag responsiveness with serum from Tnfrsf14−/− mice.
(a) Experimental protocol. Naive mice received i.v. 200 μl of serum from mice sensitized with OVA (‘OVA serum’) or mock-sensitized with PBS (‘PBS serum’) as shown in Fig. 2a, and then were challenged 24 h later with 100 μg OVA intra-tracheally (i.t.). (b,c) Lung resistance (RL) was monitored for 5 min after Ag (OVA) stimulation in mice that received (b) serum from Tnfrsf14+/+ or Tnfrsf14−/− mice or (c) serum from KitW-sh/W-sh mice engrafted i.v. with Tnfrsf14+/+ or Tnfrsf14−/− BMCMCs (in the latter mice, only MCs lacked TNFRSF14). Five-to-six female mice per group were used as indicated. Data are pooled from two independent experiments. The data in b,c (shown as mean±s.e.m.) were assessed for statistical significance using a two-way analysis of variance (ANOVA) test. Asterisks indicate statistical significance of differences between indicated groups. *P<0.05.
