Figure 2: Osteoclast-specific Gna13-CKO mice displayed osteoporosis.
(a) Quantitative reverse transcription–PCR confirmed deletion of Gna13 mRNA in day-1 Gna13f/fLysM-Cre (f/f;LysM-Cre) and day-4 Gna13f/fCtsk-Cre (f/f;Ctsk-Cre) pre-osteoclasts; N=6. (b) Western blot confirmed deletion of Gα13 protein in Gna13f/fLysM-Cre BMMs and day-5 Gna13f/fCtsk-Cre pre-osteoclasts. Quantification is co-presented in the right panels; N=6. (c) Radiographs of two-month-old wild type (WT) and Gna13f/fLysM-Cre male and female mice femurs. (d) μ-CT analysis of two-month-old WT and Gna13f/fLysM-Cre female mice femurs. (e) Quantification from d; N=6. BMD, bone mineral density; BV, bone volume; Tb.Th, trabecular bone thickness; Tb.N, trabecular bone number; Tb.Sp, trabecular bone space; TV, tissue volume. (f) TRAP staining of 2-month-old WT and Gna13f/fLysM-Cre female mice femurs. (g) High magnification images of f. (h) Quantification of N.Oc/BS (osteoclast number / bone surface) in f; N=6. (i) Radiographs of two-month-old WT and Gna13f/fCtsk-Cre female mice femurs. (j) μ-CT analysis of two-month-old WT and Gna13f/fCtsk-Cre female mice femurs. (k) TRAP staining of 2-month-old WT and Gna13f/fCtsk-Cre female mice femurs. WT, wild-type. Results were expressed as mean±s.d.; *P≤0.05; **P≤0.01; ***P≤0.001; ns, not significant (Student’s t-test). Scale bars in d,f,j,k, 200 μm; Scale bar in g, 20 μm.
