Figure 1: TRIM31 specifically inhibits NLRP3 inflammasome activation. | Nature Communications

Figure 1: TRIM31 specifically inhibits NLRP3 inflammasome activation.

From: The E3 ubiquitin ligase TRIM31 attenuates NLRP3 inflammasome activation by promoting proteasomal degradation of NLRP3

Figure 1

(a) ELISA of IL-1β, TNF-α and IL-6 in supernatants from mouse peritoneal macrophages silenced of TRIM31, primed with LPS for 8 h, and followed by stimulation with ATP, Nig. or alum for 30 min. (b) ELISA of IL-1β in supernatants from THP-1 cells transfected with TRIM31 plasmid, primed with LPS for various times, and followed by stimulation with ATP for 30 min. (c) ELISA of IL-1β, TNF-α and IL-6 in supernatants of mouse peritoneal macrophages from TRIM31+/+ or TRIM31−/− mice, primed with LPS for 8 h, and followed by stimulation with ATP, Nig., poly(dA:dT) or flagellin for 30 min. (d) Immunoblot analysis of supernatants (SN) or cell lysates (CL) from mouse peritoneal macrophages silenced of TRIM31, primed with LPS, and followed by stimulation with ATP for 30 min. (e) Immunoblot analysis of supernatants (SN) or cell lysates (CL) of mouse peritoneal macrophages from TRIM31+/+ or TRIM31−/− mice, primed with LPS, and followed by stimulation with ATP for 30 min. (f) Immunoblot analysis of supernatants (SN) or cell lysates (CL) from THP-1 transfected with TRIM31 WT or ΔRING mutant, primed with LPS, and followed by stimulation with ATP for 30 min. Similar results were obtained in three independent experiments. **P<0.01. (Student's t-test). Data are representative of three experiments (mean and s.d. of six samples in a–c).

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