Figure 6: TRIM31 deficiency enhances IL-1β secretion and aggravates Alum-induced peritonitis in vivo. | Nature Communications

Figure 6: TRIM31 deficiency enhances IL-1β secretion and aggravates Alum-induced peritonitis in vivo.

From: The E3 ubiquitin ligase TRIM31 attenuates NLRP3 inflammasome activation by promoting proteasomal degradation of NLRP3

Figure 6

(a) ELISA analysis of serum levels of IL-1β, TNF-α and IL-6 from TRIM31+/+ or TRIM31−/− mice after i.p. LPS injection. (b,c) TRIM31+/+ or TRIM31−/− deficiency mice were i.p. injected with alum for 12 h. PECs were lysed and analysed for the expression of Caspase-1, NLRP3 and ASC by immunoblot (b). Absolute numbers of neutrophils or Ly6C+ monocytes recruited to the peritoneum were analysed by fluorescence-activated cell sorting (five mice per group) (c). **P<0.01. (Student's t-test). Data are representative of three experiments (mean and s.d. of four to five samples in a and c).

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