Figure 2: Protein extraction and detection of CMCase activity.

Protein extraction and detection of CMCase activity from proteins eluted from Avicel particles after deconstruction by J1 enrichment at 90 °C for 8 days. Image shows SDS–PAGE gradient zymogram, 10–15% acrylamide, with 0.2% CMC embedded in gel. Lanes: M-marker, N-native whole SDS extract, B-buffer only soluble extract, W1-0.6% CHAPS extract, W2-1% CHAPS 5% Cellobiose extract #1 (1 h incubation at 90 °C), W-3 1% CHAPS 5% Cellobiose extract #2 (1 h incubation at 90 °C), S-1% SDS extract final wash (15 min incubation 100 °C). For lanes B through S, the Avicel pellet was sonicated continuously for 2 min in the wash solution.