Figure 4: Sequence analysis of EBI-244 enzyme.

Diagrams showing predicted signal peptide/membrane anchor (Sp), Proline threonine rich low complexity region (P/T) and structural domain architecture of EBI-244. This prediction is based on HMM modelling and searching of each putative domain, as well as multiple-sequence alignments of homologous sequences (Supplementary Fig. S3 is an example of a multiple alignment wherein domain boundaries are visible). Listed amino-acid positions are approximately predicted domain boundaries. (a) Diagram of predicted full-length native protein. (b) Recombinant EBI 244. (c) Recombinant EBI 244ΔN. (d) Amino-acid sequence of Signal peptide and P/T region.