Figure 7: HK activity is required for Src-stimulated tumour metastasis.

(a,b) The wound healing assays show that HK1/HK2 knockdown and rescue expression of their corresponding mutants weakened the migration potential of HCT116 cells. The data are expressed as the means±s.d. of three independent experiments (***P<0.001, unpaired Student’s t-test). Representative images from one experiment are shown on the left. (c,d) The transwell assays show that HK1/HK2 knockdown and rescue expression of their corresponding mutants diminished the invasion capability of HCT116 cells. Results are shown as means±s.d. of three independent experiments (***P<0.001, unpaired Student’s t-test). Representative images from one experiment are shown. (e,f) The mutation (Y732F) of c-Src phosphorylation site on HK1 disrupted tumour metastasis. In vivo metastatic assays were carried out with HCT116 cells according to the protocol shown in Methods. The data in scatter diagrams were analysed by using two-way ANOVA (n=5, **P<0.01, ***P<0.001). The scale bars in metastatic lung pictures and haematoxylin and eosin stained tissue picture are 5 and 0.5 mm, respectively.