Figure 8: HK1-p-Y732 correlates with tumour incidence and metastasis.

(a) P values and fold changes show increased expression of HK1 in various cancers as compared with normal tissue. Data are publicly available in Oncomine. (b) P value and fold changes show increased expression of HK1 in metastatic colon cancers compared with primary site tumours. (c,d) The tumour lysates from patients suffered from lung cancer (c) and oesophageal cancer (d) were detected with antibodies indicated. T, tumour; N, tumour-adjacent normal tissue. (e) IHC staining of successive colon tissue microarrays with HK1-p-Y732 antibody (left), p-c-Src antibody (middle) and HK1 antibody (right). T, Hcol-Ade carcinomas; N, tumour-adjacent normal colon tissues. IPP software was used to evaluate the intensity of the IHC staining. The data were analysed by employing Student’s t-test. Scale bars, 2 mm. (f) IHC staining of the breast tissue microarrays with HK1-p-Y732 antibody (left), p-c-Src antibody (middle) and HK1 antibody (right). T, Hbre-Duc carcinomas; N, tumour-adjacent normal breast tissues. IPP software was used to evaluate the intensity of the IHC staining. The data were analysed by using Student’s t-test. Scale bars, 2 mm.