Figure 6: Yb-bodies are assembled at the site of export of flam transcripts and fail to form foci in absence of flam transcripts

(a,e) flam transcripts (red), Armi foci (white) and nuclear membrane (green) are visualized by RNA-FISH coupled to immunofluorescence in ovarian follicle cells of ISO1A and Mago-, RnpS1-, Tsu-, Acn- and UAP56-SKD lines (a) or of Mago- and RnpS1-SKD lines (e). (b) Quantitative analysis of flam transcripts adjacent to Yb-bodies in ovarian follicle cells of these depleted lines. The percentage of cells with a nuclear or trans-membrane RNA adjacent to Armi focus is plotted. Error bars represent s.e.m. n=22 (ISO1A), n=20 (Mago), n=20 (RnpS1), n=19 (Tsu), n=18 (Acn), n=10 (UAP56), n is the number of independent experiments. (c) DNA FISH coupled to immunofluorescence labels flam DNA (green) and Armi protein in ovarian follicle cells of ISO1A and RnpS1-SKD lines. (d) Percentage of cells with Yb-bodies adjacent to flam DNA in ovarian follicle cells of RnpS1-depleted line compared with WT. Error bars represent s.e.m. n=20 (ISO1A), n=24 (RnpS1), n is the number of independent experiments. *P value <0.05, according to Mann–Whitney test. (f) Yb-bodies detected using anti-Armi antibody (red) are compared in ISO1A and homozygous flamBG flies. Anti-lamin antibody stains the nuclear periphery (green).