Figure 2: RAF1 ablation increases the number of cancer progenitor cells.
From: A cell-autonomous tumour suppressor role of RAF1 in hepatocarcinogenesis
(a) Quantification of Ki67+ liver cells 8 (top panel) or 12 weeks (bottom panel) after DEN treatment. (b) Foci of altered hepatocytes (FAH) in F/F and Δhep or Δp/np livers isolated 12 weeks after DEN injection. Sections were stained with H&E or with the indicated antibodies. FAH are delimited by dotted circles (n=3 per genotype). Scale bars, 50 μm. (c) Percentage of cancer progenitor cells (CD44+/CD31−Ter119−CD45−) present in non-aggregate and aggregate fractions of F/F and Δp/np livers, as determined by FACS analysis. Data are represented as mean±s.e.m., *P≤0.05, **P<0.01, ***P<0.005 according to Student’s t test. See also Supplementary Fig. 3.
