Figure 7: Effect of TC-specific knockdown of ANO2 on spike adaptation and mAHP currents in TC neurons. | Nature Communications

Figure 7: Effect of TC-specific knockdown of ANO2 on spike adaptation and mAHP currents in TC neurons.

From: The Ca2+-activated chloride channel anoctamin-2 mediates spike-frequency adaptation and regulates sensory transmission in thalamocortical neurons

Figure 7

(a) AHP currents were measured in VB TC neurons infected with Scr or shANO2 AAV viruses. (b) shANO2-infected neurons (n=10 from three mice) displayed significantly lower current densities compared with AAV-Scr-infected neurons (n=6 from six mice; *P=0.014, t-test). (c) Tonic firing and AHP were generated by current injection in TC neurons infected with AAV-Scr or AAV-shANO2. (d) AHP potentials in AAV-Scr- (n=6 from five mice) or AAV-shANO2-infected (n=7 from five mice) TC neurons were quantified (**P=0.009, t-test). (e,f) In shANO2-infected TC neurons, the AHP after current injection decreased, whereas the number of spikes (**P=0.009, t-test) and the adaptation indexes increased (***P=0.0008, t-test). (g) Single action potentials from AAV-Scr (black) and AAV-shANO2 (red) infected TC neurons. (h) There was no difference in the half width of single action potentials between AAV-Scr-infected (black, n=6 from five mice) and AAV-shANO2-infected (red, n=7 from five mice) TC neurons (P=0.46, t-test). (i) Representative traces of burst firing on resting membrane potential (∼−60 mV) released from hyperpolarization (∼−80 mV). (j) Patterns and number of spikes per burst were similar in AAV-Scr-infected (n=6 from five mice) and AAV-shANO2-infected (n=7 from five mice) TC neurons (P=0.57, t-test). (k) Under intact [Cl−]in conditions performed by perforated patch, tonic firing in AAV-Scr and AAV-shANO2 TC neurons were generated by 140 pA current injection. (l) An analysis of adaptation index in both AAV-Scr-infected (n=6 from three mice) and AAV-shANO2-infected (n=6 from four mice) TC neurons showed that AAV-shANO2 infection induced a significant decrease in spike-frequency adaptation (*P=0.028, t-test). (m) Tonic firing was generated by current injection in CA1 hippocampal pyramidal neurons infected with AAV-Scr or AAV-shANO2.(n) Adaptation indexes were averaged in both AAV-Scr-infected (n=9 from three mice) and AAV-shANO2-infected (n=8 from three mice) neurons (**P=0.0098, t-test). (o) Single action potentials from AAV-Scr (black) and AAV-shANO2 (red) infected CA1 neurons. (p) There was no difference in the half-width of single action potentials between AAV-Scr-infected (black, n=9 from three mice) and AAV-shANO2-infected (red, n=8 from three mice) CA1 neurons (P=0.11, t-test). Error bars represent mean±s.e.

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