Figure 8: Modulation of visceral pain responses by expression of ANO2.

(a) Timeline for behavioural testing demonstrating that tests were conducted 3 weeks after viral injection into TC neurons. Mouse brains were isolated to confirm the injection site and the efficiency of knockdown 1–2 days after behavioural testing. (b) Schematic image of the visceral pain response paradigm. (c) Representative data for the writhing response over a 10–15 min period demonstrated that AAV-shANO2 mice displayed longer and more frequent pain responses than AAV-Scr mice. (d) The number of writhing responses in mice injected with AAV-Scr (n=6) or AAV-shANO2 (n=8) was scored in 5-min intervals. VB-specific knockdown of ANO2 induced an increase in visceral pain responses in vivo (*P=0.037, **P=0.009, ***P=0.0001, t-test). Error bars represent mean±s.e.