Figure 5: GALNT14 promotes lung metastasis initiation by reversing the BMP-mediated suppression of SOX4 expression. | Nature Communications

Figure 5: GALNT14 promotes lung metastasis initiation by reversing the BMP-mediated suppression of SOX4 expression.

From: GALNT14 promotes lung-specific breast cancer metastasis by modulating self-renewal and interaction with the lung microenvironment

Figure 5

(a) Left: A Venn diagram representing the number of differentially expressed genes (P<0.05) upon knockdown and overexpression of GALNT14 in 231-LM2 (231-LM2 K/D) and in 231-Par (231-Par O/E), respectively. Right: Heat-map representation of 17 genes whose expression was decreased and increased upon knockdown of GALNT14 in 231-LM2 and overexpression in 231-Par, respectively. (b) Comparison of SOX4 transcript levels in the indicated 231-LM2 cells upon treatment with PBS or BMP4. n=6. (c) Recovery of sphere formation by GALNT14-silenced 231-LM2 cells upon restoration of SOX4 expression. Left: Comparison of sphere-forming abilities among the indicated 231-LM2 cells (VEC: control vector, SOX4: SOX4 expression vector). Right: Representative sphere images. Scale bar, 500 μm. n=6. (d) Lung colonization by cells used in c. n=9 (shCntr+VEC), 8 (shCntr+SOX4), 8 (shGALNT14+VEC) and 9 (shGALNT14+SOX4). A mouse with an unusually high-normalized photon flux of 810,000 in the shGALNT14-VEC cohort was excluded. Representative H&E staining of lung sections are shown on the right. Scale bar, 5 mm. P values based on Mann–Whitney test. Boxes: 25–75 percentiles, whiskers: 5 and 95 percentiles, line: median. P values were calculated using two-tailed unpaired Student’s t-test unless indicated otherwise. Data are mean±s.e.m. unless indicated otherwise.

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