Figure 1: Synthesis and conjugation of NPs. | Nature Communications

Figure 1: Synthesis and conjugation of NPs.

From: Tumour homing and therapeutic effect of colloidal nanoparticles depend on the number of attached antibodies

Figure 1

(a) Surfactant-coated Au NPs of 5 (5NP), 12 (12NP) and 20 (20NP) nm. (b) Synthesis scheme. Hydrophobic Au NPs are transferred from organic solvent to aqueous solution by overcoating them with an amphiphilic polymer. The surface of the polymer-coated NPs is conjugated with diamine–PEG using EDC chemistry. Using gel electrophoresis, conjugates with exactly one (5NP-1P) or two (5NP-2P) PEG molecules attached per NP are obtained. Tz antibody is oxidized with NaIO4 and linked to the free terminals of PEG, leading to NPs with one (5NP-1Tz) or two (5NP-2Tz) antibodies per NP. (c) Image of an agarose gel with NP, NP-1P and NP-2P.

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