Figure 4: Intracellular localization of 5NP-1Tz and 5NP-2Tz.

Confocal microscopy images of MCF-7 cells, incubated for 1 or 4 h at 37 °C with 65 nM of %NP-1Tz or 5NP-2Tz (FITC fluorescence in green). Early endosomes, lysosomes, Golgi and recycling endosomes were recognized, respectively, with the early endosome marker EEA1, the lysosomal protein CatD, the Golgi marker GM130 and the recycling endosome marker Tf antibodies, and labelled with an anti-mouse secondary antibody conjugated with Alexa Fluor 546 (red; Invitrogen). Nuclei were stained with DAPI (blue). The scale bar corresponds to 10 μm. Pearson’s correlation coefficients (P) have been calculated using the image J plugin JaCoP.