Figure 6: Fluorescence confocal microscopy of tumours.

Confocal laser scanning micrographs (single optical sections) of cryosections obtained from MCF-7 tumours isolated 5, 24, 48 or 96 h after exposure to 5NP-1Tz or 5NP-2Tz, labelled with AF660, and then counterstained with anti-cytokeratin19 and DAPI for tumour cells and nuclei detection, respectively. The confocal images of NPs (red) have been overlaid on the corresponding images reporting nuclei (blue) and cells (green). The scale bar corresponds to 10 μm.