Figure 2: Loss of BMP accelerates crypt regeneration and drives premalignant hyperproliferation after exposure to ionizing radiation.
From: BMP restricts stemness of intestinal Lgr5+ stem cells by directly suppressing their signature genes
(a) Representative images of Lgr5-EGFP immunofluorescence and Ki67 staining in jejunum sections from Lgr5-EGFP and Vil-CreER;Lgr5-EGFP;Bmpr1afl/fl mice at different time points after 10 Gy abdominal X-ray radiation (n=5 mice per genotype). The mice were irradiated 2 weeks following 5-day tamoxifen induction. Dotted lines delineate the proliferating epithelial invaginations on villus. (b) Quantification of crypt number and Lgr5+ stem cell number in Lgr5-EGFP and Vil-CreER;Lgr5-EGFP;Bmpr1afl/fl mice at different time points after 10 Gy abdominal X-ray radiation. Data represent mean±s.e.m. of n=5 mice per genotype. Scale bars, 50 μm.
