Figure 1: Armc5 tissue-specific expression: Armc5 mRNA expression in mice is assessed by ISH.
From: Armc5 deletion causes developmental defects and compromises T-cell immune responses
(a) Armc5 expression in adult mouse using whole-body sections. Upper panel: H/E staining; middle and bottom panels: dark field X-ray film autography with anti-sense (AS) cRNA or sense (S) cRNA as probes, respectively. Bar=1 cm. AG: adrenal gland; B: bone; BM: bone marrow; Cb: cerebellum; K: kidney; Lint: large intestine; LT: lymphatic tissue; Sk: skin; ST: stomach; Th: thymus; VB: vertebrae. (b) Armc5 expression in the adult thymus. Upper row: dark field X-ray film autography; lower row: bright field emulsion autoradiography; left column: anti-sense probe; right column: sense probe. Bars=2 mm and 20 μm. Cx: cortex; Me: medulla. (c) Armc5 expression in the adult spleen. Upper and middle panels: dark field X-ray film autography, with anti-sense and sense probes, respectively; bottom panel: bright field emulsion autoradiography. Bars=2 mm and 20 μm. WP: white pulp; RP: red pulp; CAr: central artery; Cp: capillary. (d) Armc5 mRNA in mouse spleen CD4 and CD8 cells measured by RT-qPCR. Experiments were performed three times. The results of representative experiments are shown. To facilitate comparison, normalized ratios of Armc5 versus β-actin signals (means±s.e.m.) are presented; the 0-h signal ratio of each experiment is considered as 1. (e) ARMC5 subcellular localization in L cells was detected immunofluorescence. L cells were transfected with HA-tagged mouse ARMC5-expressing construct or an empty vector, as indicated. (f) Phase contract micrographs of views in (e). The experiments were conducted three times, and micrographs of a representative experiment are shown. Scale bar: 5 μm.
