Figure 4: KO T-cell proliferation and apoptosis.
From: Armc5 deletion causes developmental defects and compromises T-cell immune responses
(a) Proliferation of spleen CD4+ and CD8+ T cells and B220+ B cells from WT and KO mice according to CFSE staining. CFSE intensity was ascertained by flow cytometry. Experiments were conducted independently 4–6 times. Representative histograms are shown. (b) Cell cycle progression of spleen T cells from WT and KO mice. The percentages of cells in G1, S and G2 phases are indicated. Experiments were conducted independently three times. Representative histograms are shown. (c) Apoptosis of WT and KO spleen T cells (gated on CD4+ plus CD8+ cells) upon FasL stimulation was determined by their annexin V expression according to flow cytometry. Experiments were conducted independently three times. Representative histograms are shown.
