Figure 8: T-cell expansion in KO mice after LCMV infection.
From: Armc5 deletion causes developmental defects and compromises T-cell immune responses
(a) Spleen CD8 cell numbers in KO mice on day 8 after LCMV infection as determined by flow cytometry. Mice number (n), means±s.e.m. and P values (two-tailed Student's t-test) are indicated. (b) Virus-specific spleen CD8 cells in KO mice on day 8 post-LCMV infection according to flow cytometry. Representative dot plots are shown. (c) Means±s.e.m. of percentages of gp33–41, np396–405 and gp276–286 tetramer-positive cells among spleen CD8 cells from all the results are presented. Numbers (n) of mice per group and P values (two-tailed Student's t-test) are indicated. (d) Means±s.e.m. of absolute numbers of gp33–41, np396–405 and gp276–286 tetramer-positive CD8 cells in the KO and WT mouse spleens on day 8 post infection. Numbers (n) of mice per group and P values (two-tailed Student's t-test) are indicated. (e,f) Memory and effector CD8 cell maturation in LCMV-infected WT and KO mice on day 8 post LCMV infection. KLRG1loCD127hi cells are considered as memory precursor effector cells (MPEC), and KLRG1hiCD127lo cells as short-lived effector cells (SLEC). Means±s.e.m. are presented. Numbers (n) of mice per group and P values (two-tailed Student's t-test) are indicated (e). Representative dot plots are shown (f). (g) On day 8 post infection, total gp33–41 np396–405 and gp276–286 tetramer-positive CD8 cells in KO and WT mouse spleen were assessed for activation markers. Means±s.e.m. are presented. Numbers (n) of mice per group and P values (two-tailed Student's t-test) are indicated.
