Figure 1: Overview of bone marrow plasma cell sampling and NGS.
From: Temporal stability and molecular persistence of the bone marrow plasma cell antibody repertoire
(a) Overview of antibody repertoire characterization method. Serial sampling of human bone marrow (BM) plasma cells over 6.5 years (left). Analysis of individual genes, gene combinations and CDR-H3s (centre) show temporally stable expression of persistent entities (right). (b) Representative fluorescence-activated cell sorting (FACS) gates of BM plasma cells (CD138+, CD38++) isolated from bone marrow mononuclear cells (BMMCs). (c) Sample collection timeline and summary of cell counts, quality-filtered sequencing reads and unique CDR-H3s for Donor 1.
