Figure 3: Postsynaptic depolarization cannot account for plasticity under EAAT2 blockade.
From: Astrocytes gate Hebbian synaptic plasticity in the striatum
(a–c) Experimental protocol (a) and averaged time-course of STDP experiments (b,c) with the recorded MSN maintained at −80 mV by intracellular current injection during the STDP pairings. LTD and LTP were induced with pairings at −70<ΔtSTDP<+70 ms (b) and ΔtSTDP=±200 ms (c), respectively. The prevention of DHK-induced depolarization did not impair the plasticity observed when MSN was depolarized. (d–f) Experimental protocol and summary of STDP experiments in which the recorded MSN was held at −50 mV (d), performed with pairings at −70<ΔtSTDP<+70 ms (e) and ΔtSTDP=±200 ms (f), respectively; in these conditions, only LTD was observed. Insets correspond to the average of 60 EPSCs during baseline and at 1 h after STDP pairings. Error bars represent the s.d. (except in bar graphs: s.e.m.). *P<0.05; **P<0.01 by one sample t-test (b,c,e,f).
