Figure 1: TGFβ stimulation of cancer cells increases SMAD7 mRNA level but not the protein level.

(a) SMAD7 mRNA expression (left), and western blot analyses of SMAD7 and EMT markers (right) after TGFβ stimulation for the indicated time duration in MCF10AT (n=3). (b) SMAD7 mRNA expression (left), and western blot analyses of SMAD7 and EMT markers (right) in the indicated cell lines after 72 h of TGFβ treatment (n=3). *P<0.05, **P<0.01, ***P<0.001 versus control by student’s t-test. Error bars are defined as s.d.