Figure 6: The role of miR-182 in bone metastasis. | Nature Communications

Figure 6: The role of miR-182 in bone metastasis.

From: MicroRNA-182 targets SMAD7 to potentiate TGFβ-induced epithelial-mesenchymal transition and metastasis of cancer cells

Figure 6

(a) Bioluminescent imaging (BLI) and X-ray of bone metastases by SCP28 cells. Arrowheads denote areas of overt osteolysis. (b) BLI quantitation of limb metastasis by SCP28 4 weeks after intracardiac injection (n=7). (c) Quantitation of osteolytic areas caused by SCP28 (n=7). (d) Representative H&E images, osteoclast TRAP staining, and IHC analyses of SMAD3 phosphorylation of bone metastases by SCP28. Arrows point to TRAP+ osteoclasts along the tumour–bone interface. Scale bars, 50 μm. (e) Osteoclast numbers along the tumour–bone interface (n=7). (f) BLI quantitation of limb metastasis by SCP28 4 weeks after intratibial injection (n=10). (g) Quantitation of osteolytic area sizes caused by SCP28 cells after intratibial injection (n=10). AU, arbitrary unit. *P<0.05, **P<0.01 by student’s t-test. Error bars are defined as s.d.

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