Figure 1: Probabilistic profiling of DDR and associated phenotypes upon mitotic perturbations.
From: Profiling DNA damage response following mitotic perturbations
(a) Probability scores were generated by LR and are displayed to show correlations between mitotic phenotypes and 53BP1-decorated DNA lesions. Values reflect scores derived from n=3 independent siRNA transfections. Each target is additionally classified according to cell proliferation (dot size is proportionate to relative cell count at the time of evaluation). Data for one of the two siRNAs (siRNA #1) for each depicted target are displayed. Numerical values for both siRNAs and all readouts are provided in Supplementary Dataset 2. (b) The same probability plot as in a where each target is additionally classified according to DNA replication (decreasing dot size reflects reduction of EdU incorporation) and p53 accumulation (heat map reflects p53 protein levels). The EdU data were derived form the same experiment as in a. Probability scores for p53 were derived from a separate experiment performed in parallel under identical conditions in n=3 independent siRNA transfections.
