Figure 2: Temporal profiling of mitotic aberrations and DDR.
From: Profiling DNA damage response following mitotic perturbations
(a) Heat maps summarizing data from representative independent time-lapse recordings (n=3) of U-2-OS cell lines stably expressing mCherry-H2B for mitotic abnormalities or mRFP-53BP1 for DNA damage after transfection with siRNAs to the indicated mitotic regulators. The phenotypes are sub-divided into three distinct temporal groups whose main characteristics are indicated. The heat maps are derived from at least 150 cells for each condition and represent fold increase values compared with mock-transfected cells. (b) Bar charts were derived from the same experiment as in a and depict average mitotic delay for each condition.
