Figure 2: DUB3 interacts with SNAIL1.
From: CDK4/6-dependent activation of DUB3 regulates cancer metastasis through SNAIL1
(a) List of SNAIL1-associated proteins identified by mass spectrometric analysis. MDA-MB-231 cells stably expressing FLAG-SNAIL1 were generated and SNAIL1 complexes were subjected to mass spectrometric analysis. (b) MDA-MB-231 cells were transfected with indicated plasmids and western blotting was performed. (c,d) MDA-MB-231 cell lysates were subjected to immunoprecipitation with control IgG, anti-SNAIL1 (c) or anti-DUB3 (d) antibodies. The immunoprecipitates were then blotted with the indicated antibodies. (e) Purified recombinant GST, GST-DUB3 and His-SNAIL1 were incubated in vitro as indicated. The interaction between DUB3 and SNAIL1 was then examined. CBS, Coomassie blue staining.
