Figure 2: SPL9 negatively regulates plant resistance to insect and JA response. | Nature Communications

Figure 2: SPL9 negatively regulates plant resistance to insect and JA response.

From: Jasmonate response decay and defense metabolite accumulation contributes to age-regulated dynamics of plant insect resistance

Figure 2

(a,b) Weight increase of H. armigera and P. xylostella larvae fed with the indicated plant leaves, both insects gained higher weight from the SPL9:rSPL9 plants. Leaves from the ∼30-day-old plants in SD were used in feeding. Data are means ±s.d. (n=25), asterisks indicate a significant difference from the wild-type (WT) group (Student’s t-test, *P<0.05, **P<0.01). (c) LOX2 and VSP2 expressions in plants over-expressing miR156 (35S:MIR156) or SPL9 (SPL9:rSPL9). Plants (30-day-old in SD) were treated with 50 μM MeJA (+JA) or ethanol (−JA) as control, and 4 h later the transcript levels in new leaves of the indicated plants were detected by qRT-PCR. The expression in the wild-type was set to 1. Data were analysed by multiple comparisons (Tukey test) followed by two-way ANOVA (*P<0.05, ***P≤0.001). Error bars represent ±s.d. (n=3). ANOVA, analysis of variance.

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