Figure 2: Biochemical localization of the T. hominis ISC pathway components.

(a) Western blots using antibodies to T. hominis proteins for fractions obtained by differential centrifugation from RK cells or RK cells infected with T. hominis (Th). Centrifugation speeds of pellet fractions are given above each lane. Sup=final 100,000g (100 K) supernatant. (b) Western blot of the 25,000g (25 K) pellet fraction of T. hominis-infected RK cells treated with or without proteinase K (PK) and Triton X-100 detergent as indicated.