Figure 2: Phenotypic comparison of Plk1-iKD and wt mice. | Nature Communications

Figure 2: Phenotypic comparison of Plk1-iKD and wt mice.

From: Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells

Figure 2

(a–c) Representative examples are shown. (a) Determination of the proliferation index in the mucosal folds of the large intestine: Ki-67-stained cells in the crypts of the transverse mucosal folds of the large intestine of a wt and a Plk1-iKD mouse. Sections are shown at two magnifications. Upper scale bars, 500 μm, lower scale bars, 50 μm. (b) Determination of the proliferation index of the ovarian follicles: Ki-67-stained cells of an ovarian follicle of a wt and of a Plk1-iKD mouse. To calculate the proliferative index in the cyclic ovary, follicles of similar sizes were selected (red boxes). Sections are shown at two magnifications. Upper scale bars, 500 μm, lower scale bars 50 μm. (c) Seminiferous tubules (stage VIII) in the testis of wt and iKD animals (primary magnification: ×20, arrows point to spermatogonia) were Bouin fixed, stained with hematoxylin/eosin or formalin fixed and stained for Ki-67 immunohistochemistry (brown nuclei of spermatogonia) with hematoxylin counterstaining. Scale bar, 50 μm. (d) Ferritin values were determined using an AU400 autoanalyzer (Olympus) in female and male Plk1-iKD mice and a sucrose-treated control group, which were all treated with Dox (means±s.e.m., n=3, for each sample). ***P<0.001, Student's t-test, unpaired and two-tailed.

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