Figure 2: Scc2 surface analysis
(a) Scc2 surface conservation with the positions of mutations indicated. (b) SCC2 mutants were tested for the ability to restore viability to a strain carrying an SCC2 degron allele. Cells of each strain were serially diluted and spotted on synthetic minimal medium without methionine (CSM–Met) plates or in plates containing indoleacetic acid (IAA). (c) Cohesin levels detected by chromatin immunoprecipitation against its Scc1 subunit followed by quantitative PCR at three cohesin-binding loci (POA1, MET10 and CEN3) and one negative control locus (TUB2) in either WT or SCC2 mutant strains arrested in mitosis. Error bars represent s.e.’s of assays each with three repeats.
