Figure 5: Functional validation of miR-221 zipper in human breast cancer cells.
From: Small RNA zippers lock miRNA molecules and block miRNA function in mammalian cells
(a) Anti-miR-221 suppressed cellular migration in MDA-MB-231 cells. Scale bar, 50 μm. (b): Quantitative analysis of A. Data are mean ±s.e.m. *P<0.05 (anti-miR-221 groups versus control groups, t-test, n=3). (c) The effects of miR-221 zipper and miR-221 Δzipper on cellular migration after transfection into MDA-MB-231 cells. The migration of living cells was recorded continuously for 48 h. Scale bar, 50 μm. (d) Quantitative analysis of C. Data are mean ±s.e.m. *P<0.05 (zipper groups versus control groups, t-test, n=3). (e) Increased miR-221 expression in Dox-resistant MCF-7 cells. Data are mean ±s.e.m. *P<0.05 (Dox. group versus control group, t-test, n=3). (f) Increased miR-221 expression in Dox-resistant MDA-MB-231 cells. Data are mean ±s.e.m. *P<0.05 (Dox. group versus control group, t-test, n=3). (g) anti-miR-221 promoted the sensitivity of MDA-MB-231 cells to 200 nM Dox. Data are mean ±s.e.m. *P<0.05 (anti-miR-221 group versus control group, t-test, n=3). (h) miR-221 zipper promoted the sensitivity of MDA-MB-231 cells to 200 nM Dox. Data are mean ±s.e.m. **P<0.01 (zipper groups versus control group, t-test, n=3).
