Figure 1: Immunoblotting and spectroscopic analysis of ΔcrtB RC/YFP–LH1 ICMs. | Nature Communications

Figure 1: Immunoblotting and spectroscopic analysis of ΔcrtB RC/YFP–LH1 ICMs.

From: Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre

Figure 1

(a) Immunoblotting with antibodies for the RC-H subunit and, separately, to YFP showing the synthesis of an RC-H/YFP polypeptide in Rba. sphaeroides. Lanes 1 and 2 indicate the ΔcrtB and ΔcrtB RC/YFP–LH1 strains, respectively. The asterisk indicates a non-specific signal from the RC-H antibodies. The numbers to the left of the anti-RC-H blot show the positions of protein standards, in kDa. (b) Room temperature and (c) 77 K absorption spectra of membranes purified from ΔcrtB (blue, dashed) and ΔcrtB RC/YFP–LH1 (red) normalized to 870 nm (b) or 880 nm (c). ΔcrtB RC/YFP–LH1 has a peak at 517 nm corresponding to YFP (indicated by arrow); this peak is shifted to 519 nm at 77 K. (d) Fluorescence excitation spectra of membranes with emission monitored at 910 nm show that YFP (peak indicated by arrow) contributes to emission at 910 nm.

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