Figure 5: Spectroscopic analysis of purified RC/YFP complexes. | Nature Communications

Figure 5: Spectroscopic analysis of purified RC/YFP complexes.

From: Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre

Figure 5

(a) Room temperature absorption spectra of control RC complexes purified from the ΔcrtB strain and RC/YFP complexes purified from the ΔcrtB RC/YFP–LH1 strain. The arrow indicates the absorption maximum of YFP at 514 nm. (b) Fluorescence spectra of purified YFP (YFP-only; red line) and the RC/YFP complex (black line) normalized to the absorbance at λexc=486 nm. (c) Normalized fluorescence decay (at 550 nm using λexc=515 nm) and fits (using two exponentials plus a constant) of YFP-only (black) and RC/YFP (red) obtained via TCSPC.

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