Figure 3: Free GOx and HRP cascade when HRP is the limiting enzyme.

(a) The time courses of ABTS+· production and the simulation curves in the presence of 1 nM GOx and 0.2 nM or 0.4 nM HRP. (b) Activity of the GOx–HRP cascade with respect to the consumption of H2O2. The assays were independently replicated twice.