Figure 4: Synthesis and the kinetics of GOx–HRP conjugate. | Nature Communications

Figure 4: Synthesis and the kinetics of GOx–HRP conjugate.

From: Proximity does not contribute to activity enhancement in the glucose oxidase–horseradish peroxidase cascade

Figure 4

(a) Synthesis of GOx–HRP conjugate. (b) SDS-PAGE analysis of the conjugate. The five lanes to the right of the standard marker correspond to native HRP, modified HRP (HRP-SMCC), native GOx, GOx–SH and the conjugate. Each lane is loaded with 1 μM protein. (c) Kinetics of HRP in the conjugate; the measurement was carried out with 1 nM conjugated HRP and 2 mM ABTS in PBS buffer. (d) Activities of free GOx, GOx–SH and the conjugated GOx. (e) Activities of free HRP, HRP-SMCC and the conjugated HRP. (f) The time courses of ABTS+· production and the model prediction based on the known activity of conjugated GOx and the Vmax and Km of conjugated HRP. (g) The differential curve of panel (f) presenting the H2O2 consumption rate. Error bars in panels (d) and (e) represent the s.d. calculated from three replicates.

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