Figure 1: Catalytic activity and localization requirements of Mon1–Ccz1. | Nature Communications

Figure 1: Catalytic activity and localization requirements of Mon1–Ccz1.

From: Architecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1–Ccz1

Figure 1

(a) Nucleotide exchange rates of Ypt7 are plotted as a function of CtMC1 concentrations. The catalytic efficiency of full-length CtMC1 and a truncated CtMC1core complex (Mon1 195–355, Ccz1 1–249) are comparable. Error bars represent s.d. of three independent biological repeats. (b) GFP-tagged truncations of ScMon1 and (c) ScCcz1 are introduced into mon1Δ and ccz1Δ yeast knockout strains. The N-terminus of Mon1 is dispensable, but deletion of the Mon1 and Ccz1 C-termini cause mislocalization and vacuolar fragmentation. (d) The C-terminus of Mon1 is replaced by a PI3P-binding FYVE domain and artificially recruited to endosomal and vacuolar membranes as seen in a wild-type background, but Mon1-ΔC-FVYE is not able to complement a mon1Δ strain. Scale bars: 5 μm.

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